Every antibody campaign we run — now on one menu
From de novo discovery to picomolar lead engineering, browse the exact service your project needs. Each one is a workflow we've actually run, with the data to prove it. Flexible terms, no downstream royalties.
Pick your starting point
Have only a target? Start in Discovery. Already have a lead sequence? Jump to Engineering. Need a hard-to-make specificity — phospho, hapten, glycan, anti-idiotype? Those are in Discovery too. Every service below is scoped individually and quoted on request.
No sequence, no antibody yet. We raise a brand-new mAb or VHH from your target antigen, cells, or tissue using patented WizAmp™ phage display.
Rabbit mAb Discovery (RabWiz™)
De novo rabbit monoclonals for your toughest targets.
What we do
We immunize rabbits and build an immune phage library of 5×109–1010 with our patented WizAmp™ method, then pan against recombinant protein, peptide, or native/transfected cells. Our proprietary phagemid vector optimally expresses and displays rabbit Fab whose CK1 light chains carry extra cysteines in FR3L and CK1 (about 90% of rabbit light chains), and its dual-purpose design lets us screen soluble Fab in 96-well plates before the costly IgG stage. Rabbit repertoires carry unusually high CDR diversity in both heavy and light chains, plus high intrinsic affinity, so high-function clones often come from just a few germlines.
You receive
- Sequence-confirmed clones (full VH/VL)
- ELISA, flow, and BLI binding data (optional)
- Recombinant IgG
- No downstream royalties
See the data →Rabbit phage display accesses rare functional antibodiesRequest a QuoteVHH / Single-Domain (Nanobody) Discovery
Camelid single-domain antibodies for hidden epitopes.
What we do
We immunize camelids and pan a VHH library. Our proprietary phagemid efficiently expresses and selects VHHs with extra-long HCDR3 and cysteines in HCDR2/HCDR3. Their small single domain reaches clefts, catalytic sites, and conserved epitopes that conventional Fab/IgG cannot. Selected VHHs can be reformatted to VHH-Fc or bivalent constructs.
You receive
- VHH sequences
- BLI binding data (optional)
- VHH-Fc / format conversion
Membrane & Difficult-Antigen Discovery
Cell-based panning for targets you cannot express as a recombinant protein.
What we do
When a target cannot be purified or expressed as a recombinant protein, we immunize with peptides and/or cells and pan directly on native or transfected cells, eliminating clones that bind negative cells so selection sees the folded, post-translationally modified epitope. That yields functional clones recombinant antigen cannot.
You receive
- Cell-binding-confirmed clones
- Flow cytometry data
See the data →Antibodies to multi-transmembrane proteins by peptide immunizationRequest a QuoteFunctional / Neutralizing Antibody Discovery
Find the clones that actually move your function.
What we do
Useful clones often bind well beyond the single dominant epitope, so we recover a broad, diverse panel and cluster it by HCDR3 similarity to surface functionally distinct lineages. We then work with you to test those lineages and home in on the clones that drive the function you need. Non-functional clones are not wasted — we can use them to mask dominant epitopes and isolate antibodies to novel epitopes, and for ligand–receptor blockers we use the receptor or ligand itself as a reagent to select clones that interfere with binding. For SARS-CoV-2 we iteratively re-matured leads as new variants emerged, driving broad neutralization to low ng/mL EC50 even on subvariants the parent antibody no longer bound.
You receive
- Diverse clone panel, clustered by HCDR3 lineage
- Sequences and binding data; functional/neutralization testing by arrangement
See the data →Mechanism-guided panning reveals a hidden neutralizing lineageRequest a QuoteSmall-Molecule / Hapten Antibody
Antibodies to targets too small to be immunogenic — even in samples full of cross-reactive look-alikes.
What we do
Small molecules are not immunogenic on their own, so we start with hapten design and a carrier-conjugation strategy. After immunization we pan the library in the presence of the soluble cross-reactive substances found in real samples, absorbing cross-reactive binders (competitive selection), then confirm specificity by competition ELISA with free analyte. Our real strength is steering selection away from structurally similar molecules, so the antibody cleanly discriminates your analyte from close structural analogs — the hardest part of any small-molecule immunoassay.
You receive
- Small-molecule-specific clones
- Competition-ELISA specificity data
See the data →Antibody to a small-molecule oxidative-stress marker (8-OHdG)Request a QuoteAnti-Idiotype Antibody
Antibodies that recognize your drug's variable region.
What we do
We raise antibodies that recognize only the idiotype of your antibody drug — panning on the drug while subtracting on irrelevant IgG to guarantee idiotype specificity. We support the full range of anti-id types, including antibodies that bind only the drug–antigen complex, free-drug-specific, and drug-captured formats, with a deep track record across all of them.
You receive
- Idiotype-specific clones (type of your choice)
- Free vs. complex discrimination data
See the data →Anti-idiotype antibodies for therapeutic drug monitoringRequest a QuotePhospho / PTM-Specific Antibody (pS-Wiz)
Monoclonals specific to a single phospho-site.
What we do
You supply the synthetic phospho-peptide (and unmodified control); we pan our pS-Wiz synthetic phage-display library and use subtractive selection to isolate clones that bind the phosphorylated form but not the unmodified peptide — or an irrelevant phospho-peptide, avoiding pan-phospho-serine antibodies. pSer and pThr supported. You pay only for specificity-confirmed clones, and your sequences stay confidential.
You receive
- Phospho-specific clones (WB/flow validation available)
- Phospho vs. non-phospho specificity data
See the data →Cell panning selects phosphorylation-specific rabbit mAbsRequest a QuoteCarbohydrate / Glycan & Sulfation Antibody
Specificity against sugars, glycans, and sulfation.
What we do
Glycans, polysaccharides, and sulfotyrosine are harder to target than protein epitopes. We use conjugated antigens plus subtractive panning to isolate specific clones, subtracting cross-reactivity to related glycans. In a published CCR5 study our sulfation-site-specific mAb bound the fully sulfated N-terminal peptide at 0.81 nM KD with no measurable binding to the non-sulfated peptide, and recognized full-length sulfated CCR5 on the cell surface.
You receive
- Glycan / PTM-specific clones
- Cross-reactivity data
See the data →Site-specific antibodies for sulfated GPCR N-termini (CCR5, J Biol Chem 2025)Request a QuoteSend us an amino-acid sequence. We re-engineer it for affinity, kinetics, specificity, developability, or format — with benchtop selection, not in-silico guesses alone.
Affinity Maturation (STEM™)
Drive your lead into picomolar KD.
What we do
Three-stage STEM™: Stage A1 selects single-CDR variant libraries mildly to collect functional clones; A2 combines light- and heavy-chain CDR pools separately under stringent selection; Stage B merges both chains into one library under the most stringent conditions for the highest-affinity binders. CDRs are designed from human antibody amino-acid usage — avoiding unfavorable residues at each position — using a local AlphaFold instance plus germline/structural bioinformatics, and every stage carries thermostability and polyreactivity filters. Reaches pM even from low-nM starts.
You receive
- BLI binding data (optional, after IgG purification)
- Full sequences
- Recombinant IgG
See the data →STage-Enhanced Maturation (STEM) technologyRequest a QuoteAffinity Attenuation / De-tuning
Deliberately dial affinity down into a target window.
What we do
We select the combined library across all fast/slow on- and off-rate conditions, capturing stronger and weaker clones simultaneously, then narrow to the affinity window your application needs.
You receive
- Sequences of the affinity-variant clone panel
- Ranking of the panel by binding strength (ELISA)
See the data →STEM affinity-engineering examplesRequest a QuoteAvidity Engineering
Separate monovalent affinity from avidity.
What we do
We identify high-affinity candidates by monovalent ELISA and, separately, weak-but-high-avidity candidates by bivalent ELISA (Fab pre-complexed with secondary to form pseudo-IgG). This lets you design how monovalent vs. multivalent binding drives your function.
You receive
- Monovalent/bivalent characterization
- Avidity-binned clone panel
See the data →Avidity engineering: monovalent Fab vs bivalent pseudo-IgGRequest a QuoteKinetic Tuning (on-/off-rate)
Target on-rate and off-rate independently.
What we do
Our in-vitro selections can target on-rate and/or off-rate independently — engineering fast-off or slow-on profiles by designing wash time and competition into the selection.
You receive
- on/off-rate kinetic data
- Clones matched to your target kinetics
pH-Sensitivity / Recycling Engineering
Antibodies that release antigen at pH ~6.
What we do
We select clones that bind at pH 7.4 but release rapidly at pH ~5.8 — for endosomal recycling or the acidic tumor microenvironment. Starting from a pH-insensitive anti-TNFα parent, we engineered pH-sensitive clones and confirmed pH 7.4 binding / pH 5.8 dissociation by Octet.
You receive
- pH-dependent binding/dissociation data (Octet)
Thermostability Engineering
Select for heat-stable clones under stress.
What we do
We transiently heat and cool the phage library to drop unstable clones and enrich thermoresistant ones, raising stringency each stage so clones retain strong biophysical properties after IgG conversion.
You receive
- Thermostability data
- Developability profile
Developability Optimization
Strip out aggregation, viscosity, and liabilities.
What we do
Aggregation, high-concentration viscosity, sequence liabilities, PTM susceptibility and polyreactivity all hurt developability. With STEM™ antibody engineering we expand clone diversity and then select the variants with good biophysical properties, improving developability while keeping function.
You receive
- Full developability data set
- Scale-up suitability assessment
See the data →Developability assessment and improvementRequest a QuoteExpression / Titer Rescue
Fix a functional antibody that will not express.
What we do
With STEM™ antibody engineering we expand clone diversity and select variants that keep function but express far better. In one program this reached more than 10× higher yield with retained function (therapeutic patent filed).
You receive
- Expression titer data
- Parent-equivalent function data
Cross-Species Reactivity Engineering
Add cyno / mouse cross-reactivity to a lead.
What we do
During humanization/maturation we add cross-reactivity to cyno, mouse, or other orthologs of the same antigen — avoiding the need to raise a separate surrogate antibody.
You receive
- Cross-species binding data
Mouse mAb Humanization
Humanize a mouse lead without losing function.
What we do
We graft mouse CDRs onto a human acceptor germline framework and restore binding-critical Vernier/interface residues by back-mutation. With STEM™ we further humanize the CDRs themselves while keeping function and lowering immunogenicity.
You receive
- Humanized IgG (~1 mg)
- ELISA and kinetic data
- Full VH/VL sequences
See the data →Humanization capability and experienceRequest a QuoteRabbit mAb Humanization
Humanize high-affinity rabbit leads (our home turf).
What we do
Rabbit mAbs usually derive from a few germlines with well-defined canonical CDRs, so humanization is comparatively straightforward. We apply RabWiz/STEM know-how to humanize while preserving affinity and specificity — routine in our own therapeutic pipeline.
You receive
- Humanized IgG
- Binding/function data
- Full sequences
Llama / Alpaca (VHH) Humanization
Humanize camelid VHH domains.
What we do
We substitute the VHH hallmark framework residues with human equivalents while preserving the solubility and stability that make single domains attractive, using STEM™ to retain function.
You receive
- Humanized VHH
- Solubility/stability data
Chimeric Antibody Generation
Fuse your variable region to human constant.
What we do
We fuse the source variable region to a human IgG constant region — useful for a quick function/isotype check before full humanization, or for secondary-reagent compatibility.
You receive
- Chimeric IgG
- Function-confirmation data
Feline / Veterinary Antibody Engineering
Engineer antibodies for animal-health targets.
What we do
We have affinity-matured and engineered antibodies across animal species including feline, supporting veterinary therapeutic and diagnostic leads.
You receive
- Species-specific maturation/optimization data
How an engagement works
Scope the campaign
Tell us your target or send your sequence. We define deliverables, timeline, and success criteria up front.
Design & build
AI-guided CDR design plus WizAmp™ library construction, diversity up to >1010.
Select & screen
Phage panning with stringency, stability and polyreactivity filters. Fabs screened in 96-well before IgG.
Characterize & deliver
ELISA, flow, and BLI data (optional), plus full sequences and clone data.
Frequently asked questions
What antibody discovery services does Abwiz Bio offer?
Abwiz Bio provides de novo discovery of rabbit monoclonal antibodies (RabWiz™), VHH / single-domain antibodies, membrane and difficult-antigen antibodies via cell-based panning, and functional/neutralizing antibodies. We also specialize in hard-to-raise specificities: phospho/PTM, anti-idiotype, hapten/small-molecule, and glycan antibodies. Every campaign uses our patented WizAmp™ phage display platform.
How much affinity improvement can affinity maturation achieve?
Our STEM™ platform has delivered up to 1,300-fold affinity improvement, reaching KD as low as 4 pM even when starting from low-nanomolar leads. Because we pair AI-guided library design with benchtop directed evolution, each engineered clone also passes stability and polyreactivity filters, so it is developability-ready.
How do IP and commercial terms work?
IP and commercial terms are flexible and agreed before the project starts, and our engagements carry no downstream royalties. We tailor terms to your program rather than applying a one-size-fits-all model.
Can you humanize mouse, rabbit or VHH antibodies?
Yes. We humanize mouse, rabbit, and llama/alpaca (VHH) antibodies by CDR grafting onto human germline frameworks, then restore affinity through Vernier-residue back-mutation and, when needed, STEM™ affinity maturation. Our humanization success rate is 15/15.
What data do I receive at the end of a project?
Deliverables depend on the service but typically include full VH/VL sequences, recombinant IgG or VHH, and characterization by ELISA and flow cytometry, with BLI binding data optional after IgG purification, plus functional assay data on request.
Can I start mid-workflow if I already have a lead antibody?
Absolutely. Discovery, humanization, and each engineering service can be run standalone. If you already have a lead, send the amino-acid sequence and we begin directly at affinity maturation, developability, expression rescue, or format engineering.
The science behind the menu
Representative data and workflows from our own campaigns. Click any panel to enlarge.
Rabbit phage display accesses rare functional antibodies
WizAmp™ enables superior antibody library construction
Efficient selection of rabbit CK1 Fab clones
Mechanism-guided panning reveals a hidden neutralizing lineage
Broadly neutralizing anti-Omicron antibodies via rabbit + STEM™
Cell panning selects phosphorylation-specific rabbit mAbs
Antibodies to multi-transmembrane proteins by peptide immunization
Point-mutation (H1047R)-specific antibody development
Antibody to a small-molecule oxidative-stress marker (8-OHdG)
Anti-idiotype antibodies for therapeutic drug monitoring
Anti-idiotype Fab that only binds the drug–antigen complex
Site-specific antibodies for sulfated GPCR N-termini — CCR5 (J Biol Chem 2025)
STage-Enhanced Maturation (STEM™) technology
STEM™ stage-by-stage selection flow
STEM™ affinity-engineering examples
Affinity maturation ELISA — stage-by-stage improvement
Avidity engineering: monovalent Fab vs bivalent pseudo-IgG
Humanization capability and experience
Humanization, potency & developability optimization with STEM™
Developability assessment and improvement
Post-humanization STEM™: potency, developability & pH sensitivity
Not sure which service fits?
Send us your target or your sequence. A scientist — not a sales rep — will tell you the fastest route to the antibody you need, and quote it.
Request a Quote
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