Rabbit monoclonals for the targets that defeat everyone else.
GPCRs, ion channels, phospho-sites, anti-idiotypes, single small molecules — the antibodies that hybridoma and B-cell screening quietly miss. RabWiz™ pairs the rabbit immune response with phage-display selection to find the rare, functional clone others can't reach.
You can only screen antibodies your method actually captured.
The best antibody against your target is often a rare clone hiding on a minor epitope. If the discovery method never captured it, no amount of screening brings it back — and you'll never know it was there. Here's how the common approaches compare.
| Hybridoma | B-cell cloning | RabWiz™ (Abwiz) | |
|---|---|---|---|
| Repertoire captured | 10⁴–10⁵ cells | 10⁴–10⁵ cells | Up to 2×10¹⁰ — full immune diversity |
| Rare / minor-epitope clones | Often lost | Often lost | Captured & selectable |
| Remove cross-reactive clones | No | No | Yes — competitive selection |
| Rabbit Ck1 clones (70–90% of serum) | Partial | Partial | Fully accessible (engineered vector) |
| Re-screen after new target insight | One-shot | One-shot | Libraries archived — re-select anytime |
| Native H/L chain pairing | Yes | Yes | Yes — retained through cloning |
Three advantages, one integrated platform.
Why rabbits
Rabbit CDRs are more diverse than human or mouse — and their light chains nearly as diverse as heavy chains. The result is exquisite specificity and typically picomolar affinity, plus responses to targets that are invisible to the mouse immune system.
Why phage display
Access libraries up to 2×10¹⁰ and apply selection pressure hybridoma can't: competitive ELISA to pull single-molecule specificity, subtractive panning on cells, and epitope-masking to force neutralizing clones out of hiding.
Why only Abwiz
Rabbit Ck1 clones — 70–90% of the serum response — carry extra cysteines and historically wouldn't express in E. coli. We engineered a phagemid vector that displays them. Other platforms fall back to scFv or chimeric constructs and miss that majority of the repertoire.
Immunization to validated IgG in ~4–6 months.
Immunization
- Antigen preparation
- Rabbit immunization
- Serum analysis
Library construction
- WizAmp™ gene cloning
- Optimized rabbit-Fab phagemid
- QC report
Panning / screening
- Phage panning
- Fab ELISA / flow
- Full VH/VL sequences
IgG production
- HEK293 expression
- ~1 mg purified IgG
- Validation + affinity
Published proof on hard targets.
Over 400 libraries against hundreds of targets. A few examples where RabWiz™ selection reached antibodies conventional screening can't.
Antibody that reads a single sulfation site on CCR5
Sulfated-peptide immunization → WizAmp™ immune Fab library → counter-selection with CCR5 / TPST cell screening. The clone (BA8) tells apart sulfation state and site, and binds full-length CCR5 on the cell surface.
A 1-picomolar nanobody to a flexible loop others can't reach
The same WizAmp™ + phagemid vector that displays cysteine-rich rabbit Ck1 Fabs is ideal for cysteine-rich VHH libraries. Against complement factor H, it delivered functional clones with unusually long, disulfide-stabilized CDR3s.
A neutralizing lineage engineered to keep pace with variants
From Wuhan-Hu-1 RBD immunization to a parental rabbit clone (C-A11), humanized (hN2Y) and then STEM™-matured to broadly neutralize Omicron sublineages — validated by CoVIC at La Jolla Institute.
Anti-idiotype antibodies
Both Type 1 (drug-antibody specific) and Type 2 (drug/antigen-complex only) anti-idiotypes for PK assays and immunogenicity controls — selected by competitive phage panning.
Native membrane targets
Functional mAbs against CB1, CB2, PAR1 and the ion channel Kv1.3 — all recognizing the native, cell-displayed receptor, using peptide, ECL-Fc, cell and RNA formats.
Single-molecule discrimination
A rabbit mAb specific to an oxidized nucleoside with no reactivity to near-identical small molecules — reachable only through competitive selection, not screening.
Phospho-sites and cell immunization
Phospho-specific mAbs that hold up in WB/IHC/flow via paired peptide + native-cell panning; and cancer-cell-specific clones from subtractive panning on primary cells — re-selectable from archived libraries.
Not just rabbit — the same library technology extends across formats.
WizAmp™ uniform single-primer amplification and our cysteine-tolerant phagemid vector aren't rabbit-specific. The approach that captures a full rabbit repertoire applies to VHH, mouse and human antibody development — with matching humanization and STEM™ affinity maturation in the same pipeline.
Rabbit discovery & engineering
Our core platform: high-affinity, highly specific rabbit mAbs against difficult targets, then humanization and maturation as needed.
Camelid VHH libraries
The same cysteine-tolerant vector displays cysteine-rich VHH libraries that conventional phagemids can't — ideal for nanobody discovery.
Mouse & chimeric engineering
Immune-library discovery and STEM™ engineering applied to mouse-derived and chimeric antibodies — potency and developability, not just binding.
Human & humanized leads
Humanization to human frameworks and STEM™ affinity maturation of human and humanized therapeutic leads — ready to hand off for development.
Common questions.
Should we use rabbit or mouse antibodies?
How is this different from hybridoma or B-cell cloning?
Do rabbit Fabs really work in phage display?
Can you humanize the rabbit mAb afterward?
Are the heavy and light chains natively paired?
Have a target that's beaten other platforms?
That's exactly what RabWiz™ was built for. Tell us the antigen and the assay it needs to work in — we'll tell you whether we can reach it.
Get in touch